CNN1 Antikörper (CF®594)

Produktnummer ABIN6178198

Der Maus Monoklonal Anti-CNN1-Antikörper wurde für FACS, IF und IHC (f) validiert. Er ist geeignet, CNN1 in Proben von Human und Ratte zu detektieren.

Kurzübersicht für CNN1 Antikörper (CF®594) (ABIN6178198)

Target: CNN1 (Calponin 1 (CNN1))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CNN1 Antikörper ist konjugiert mit CF®594

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: CALP CNN1-832

Produktdetails anti-CNN1 Antikörper (CF®594)

Verwendungszweck: Mouse Monoclonal anti-Calponin-1 (CALP + CNN1/832), CF594 Conjugate

Produktmerkmale: Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human CNN1 protein (CNN1/832), Crude human uterus extract (CALP)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Myoepithelial cells in breast ducts or uterus

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu CNN1

Target: CNN1 (Calponin 1 (CNN1))

Andere Bezeichnung: Calponin-1

Hintergrund: Calponin 1 basic smooth muscle, Calponin H1 smooth muscle, Calponin-1, CNN1, Cnn1, Sm Calp, SMCC

Molekulargewicht: 34 kDa

Gen-ID: 1264, 465929

UniProt: P51911