CD20 Antikörper (CF®647)

Produktnummer ABIN6176206

Der Maus Monoklonal Anti-CD20-Antikörper wurde für FACS, IF und Func validiert. Er ist geeignet, CD20 in Proben von Human zu detektieren.

Kurzübersicht für CD20 Antikörper (CF®647) (ABIN6176206)

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CD20 Antikörper ist konjugiert mit CF®647

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Functional Studies (Func)

Klon: 93-1B3

Produktdetails anti-CD20 Antikörper (CF®647)

Verwendungszweck: Mouse Monoclonal anti-CD20 (93-1B3), CF647 Conjugate

Produktmerkmale: This antibody recognizes a protein of 30-33 kDa, which is identified as CD20 (Workshop V, Code CD20.4). It is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. The protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B-cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as calcium channel involved in B cell activation and cell cycle progression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Stimulated human leukocytes

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Functional Studies order Ab without BSA & azide)
• Optimal dilution for a specific application should be determined by user

Kommentare:

Daudi, Raji, and U266, and human lymphocytes. Lymph nodes and tonsils.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu CD20

Target: CD20 (MS4A1) (Membrane-Spanning 4-Domains, Subfamily A, Member 1 (MS4A1))

Andere Bezeichnung: CD20

Molekulargewicht: 33-37 kDa

Gen-ID: 931, 712553

UniProt: P11836