MLANA Antikörper

Produktnummer ABIN6173347

Der Maus Monoklonal Anti-MLANA-Antikörper wurde für WB, IF, FACS, ISt und IHC (f) validiert. Er ist geeignet, MLANA in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für MLANA Antikörper (ABIN6173347)

Target: MLANA (Melan A (MLANA))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MLANA Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunostaining (ISt), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: M2-9E3

Produktdetails anti-MLANA Antikörper

Verwendungszweck: Mouse Monoclonal anti-MART-1 / Melan-A (M2-9E3), Purified, BSA-free

Produktmerkmale: This antibody recognizes a protein doublet of 20-22 kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Recombinant hMART-1 protein

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Konservierungsmittel: Azide free

Details zu MLANA

Target: MLANA (Melan A (MLANA))

Andere Bezeichnung: MART-1 / Melan-A

Hintergrund: Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA

Molekulargewicht: 20-22 kDa (doublet)

Gen-ID: 2315, 154069

UniProt: Q16655