Rekombinanter ANO1 Antikörper
Kurzübersicht für Rekombinanter ANO1 Antikörper (ABIN6173155)
Target
Alle ANO1 Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Verwendungszweck
- Recombinant Rabbit Monoclonal anti-DOG-1 / TMEM16A (DG1/1487R), Purified, with BSA
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Produktmerkmale
- Expression of DOG-1 protein is elevated in the gastrointestinal stromal tumors (GIST s), c-kit signaling-driven mesenchymal tumors of the GI tract. DOG-1 is rarely expressed in other soft tissue tumors, which, due to appearance, may be difficult to diagnose. Immunoreactivity for DOG-1 has been reported in 97.8 percent of scorable GIST s, including all c-kit negative GIST s. Overexpression of DOG-1 has been suggested to aid in the identification of GISTs, including Platelet-Derived Growth Factor Receptor Alpha mutants that fail to express c-kit antigen. The overall sensitivity of DOG1 and c-kit in GIST s is nearly identical: 94.4 % vs. 94.7 %. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
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Aufreinigung
- Purified
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Immunogen
- Recombinant full-length human DOG-1 protein
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Isotyp
- IgG kappa
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Applikationshinweise
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Immunohistology (formalin) 0.25-0.5 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Optimal dilution for a specific application should be determined by user
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Kommentare
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Gastrointestinal Stromal Tumor (GIST) or testicular germ cell tumor. Melanocytes in the basal layer of the epidermis and mast cells in the dermis of normal skin.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konzentration
- 200 μg/mL
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Buffer
- PBS/0.05 % BSA/0.05 % azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- ANO1 (Anoctamin 1, Calcium Activated Chloride Channel (ANO1))
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Andere Bezeichnung
- DOG-1 / TMEM16A
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Hintergrund
- Anoctamin 1, Calcium Activated Chloride Channel, Discovered On Gastrointestinal Stromal Tumors Protein 1, TAOS2, ORAOV2, TMEM16A
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Molekulargewicht
- ~114 kDa
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Gen-ID
- 55107, 503074
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UniProt
- Q5XXA6
Target
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