Keratin 10 Antikörper (CF®594)

Produktnummer ABIN6172384

Der Maus Monoklonal Anti-Keratin 10-Antikörper wurde für FACS, IF und IHC (f) validiert. Er ist geeignet, Keratin 10 in Proben von Human und Maus zu detektieren.

Kurzübersicht für Keratin 10 Antikörper (CF®594) (ABIN6172384)

Target: Keratin 10 (KRT10)

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Keratin 10 Antikörper ist konjugiert mit CF®594

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: KRT10-844

Produktdetails anti-Keratin 10 Antikörper (CF®594)

Verwendungszweck: Mouse Monoclonal anti-Cytokeratin 10 (KRT10/844), CF594 Conjugate

Produktmerkmale: This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human KRT10 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.1-0.2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

A431, HeLa, MCF7 cells. Esophagus or tonsil.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Keratin 10

Target: Keratin 10 (KRT10)

Andere Bezeichnung: Cytokeratin 10

Hintergrund: BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10

Molekulargewicht: 56.5 kDa

Gen-ID: 3858, 99936

UniProt: P13645