Keratin 10 Antikörper (CF®568)
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- Target Alle Keratin 10 (KRT10) Antikörper anzeigen
- Keratin 10 (KRT10)
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser Keratin 10 Antikörper ist konjugiert mit CF®568
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Applikation
- Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)
- Verwendungszweck
- Mouse Monoclonal anti-Cytokeratin 10 (KRT10/844 + KRT10/1275), CF568 Conjugate
- Produktmerkmale
- This MAb recognizes a protein of 56.5 kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation, it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human KRT10 protein
- Klon
- KRT10-844 KRT10-1275
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product KRT10 Primärantikörper
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- Applikationshinweise
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Flow cytometry 0.5-1 μg/million cells in 0.1 mL
- Immunohistology (formalin) 0.1-0.2 μg/mL
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min
- Immunofluorescence 0.5-1 μg/mL
- Optimal dilution for a specific application should be determined by user
- Kommentare
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A431, HeLa, MCF7 cells. Esophagus or tonsil.
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Protect from light
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- Target
- Keratin 10 (KRT10)
- Andere Bezeichnung
- Cytokeratin 10 (KRT10 Produkte)
- Synonyme
- BCIE antikoerper, BIE antikoerper, CK10 antikoerper, EHK antikoerper, K10 antikoerper, KPP antikoerper, D130054E02Rik antikoerper, K1C1 antikoerper, Krt-1.10 antikoerper, Krt1-10 antikoerper, Ka10 antikoerper, Ker10 antikoerper, KRT10 antikoerper, KRT25 antikoerper, KRT27 antikoerper, KRT28 antikoerper, keratin 10 antikoerper, keratin 10, type I S homeolog antikoerper, keratin, type I cytoskeletal 25 antikoerper, KRT10 antikoerper, Krt10 antikoerper, krt10.S antikoerper, LOC101085587 antikoerper
- Hintergrund
- BCIE, BIE, EHK, Keratin Type I Cytoskeletal 10, KRT10
- Molekulargewicht
- 56.5 kDa
- Gen-ID
- 3858, 99936
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