Desmin Antikörper (Biotin)

Produktnummer ABIN6171211

Der Maus Monoklonal Anti-Desmin-Antikörper wurde für WB, IHC, IF und FACS validiert. Er ist geeignet, Desmin in Proben von Human, Ratte, Maus, Huhn und Hamster zu detektieren.

Kurzübersicht für Desmin Antikörper (Biotin) (ABIN6171211)

Target: Desmin (DES)

Reaktivität: Human, Ratte, Maus, Huhn, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: DES-1711

Produktdetails anti-Desmin Antikörper (Biotin)

Verwendungszweck: Mouse Monoclonal anti-Desmin (DES/1711), Biotin Conjugate

Produktmerkmale: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain ?-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. The antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length desmin protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 1-2 μg/mL

• Immunofluorescence 1-2 μg/mL Flow cytometry 0.5-1 μg/million cells
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

SJRH30 Cells. Uterus or Leiomyosarcoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Desmin

Target: Desmin (DES)

Andere Bezeichnung: Desmin

Hintergrund: CMD1I, CSM1, CSM2, DES, Intermediate filament protein

Molekulargewicht: 52 kDa

Gen-ID: 1674, 594952

UniProt: P17661