Myogenin Antikörper (AA 30-224) (CF®594)

Produktnummer ABIN6171054

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Myogenin in IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Schwein und Cat.

Kurzübersicht für Myogenin Antikörper (AA 30-224) (CF®594) (ABIN6171054)

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reaktivität: Human, Maus, Ratte, Schwein, Cat

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Myogenin Antikörper ist konjugiert mit CF®594

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: MGN185 F5D

Produktdetails anti-Myogenin Antikörper (CF®594)

Bindungsspezifität: AA 30-224

Verwendungszweck: Mouse Monoclonal anti-Myogenin (MGN185 + F5D), CF594 Conjugate

Produktmerkmale: Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80 % amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms? tumor. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Human myogenin recombinant protein (MGN185), Rat myogenin recombinant fragment containing amino acid 30-224 (F5D)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Myogenin

Target: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Andere Bezeichnung: Myogenin

Hintergrund: BHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4

Molekulargewicht: 34 kDa

Gen-ID: 4656, 2830

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development