Fibronectin Antikörper (Biotin)

Produktnummer ABIN6168703

Der Maus Monoklonal Anti-Fibronectin-Antikörper wurde für IF, FACS und IHC (f) validiert. Er ist geeignet, Fibronectin in Proben von Human, Ratte, Maus und Schwein zu detektieren.

Kurzübersicht für Fibronectin Antikörper (Biotin) (ABIN6168703)

Target: Fibronectin

Reaktivität: Human, Ratte, Maus, Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Fibronectin Antikörper ist konjugiert mit Biotin

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: 2755-8

Produktdetails anti-Fibronectin Antikörper (Biotin)

Verwendungszweck: Mouse Monoclonal anti-Fibronectin (2755-8), Biotin Conjugate

Produktmerkmale: Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblasts phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren's disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: T-cell lymphoma biopsy

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

SW156 cells or Kidney

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Fibronectin

Target: Fibronectin

Hintergrund: Cold insoluble globulin (CIG), FINC, FN1, FNZ, GFND, GFND2, LETS, Migration stimulating factor (MSF), Ugl-Y3

Molekulargewicht: 220 kDa (monomer), 440 kDa (dimer)

Gen-ID: 2335, 203717

UniProt: P02751