UCHL1 Antikörper (CF®488A)

Produktnummer ABIN6168295

Der Maus Monoklonal anti-UCHL1 Antikörper wird verwendet zum Nachweis von UCHL1 in Proben von Human, Ratte, Maus, Rind (Kuh) und Schwein. Er wurde validiert für WB und IHC (f).

Kurzübersicht für UCHL1 Antikörper (CF®488A) (ABIN6168295)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser UCHL1 Antikörper ist konjugiert mit CF®488A

Applikation: Western Blotting (WB), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: 31A3

Produktdetails anti-UCHL1 Antikörper (CF®488A)

Verwendungszweck: Mouse Monoclonal anti-PGP 9.5 / UCHL-1 (31A3), CF488A Conjugate

Produktmerkmale: This MAb reacts with a protein of 20-30 kDa, identified as PGP 9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UCHL-1). Initially, PGP 9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP 9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP 9.5 gene is believed to cause a form of Parkinson's disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Native UchL1 (PGP 9.5) protein from brain

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Cerebellum

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Andere Bezeichnung: PGP 9.5 (UCHL-1)

Molekulargewicht: 20-30 kDa

Gen-ID: 7345, 518731

UniProt: P09936

Pathways: Feeding Behaviour