Transgelin Antikörper (CF®647)

Produktnummer ABIN6167711

Dieses Anti-Transgelin-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Transgelin in FACS und IF. Geeignet für Human, Maus, Kaninchen, Rind (Kuh) und Schwein.

Kurzübersicht für Transgelin Antikörper (CF®647) (ABIN6167711)

Target: Transgelin (TAGLN)

Reaktivität: Human, Maus, Kaninchen, Rind (Kuh), Schwein

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Transgelin Antikörper ist konjugiert mit CF®647

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: TAGLN-247

Produktdetails anti-Transgelin Antikörper (CF®647)

Verwendungszweck: Mouse Monoclonal anti-Transgelin / SM22-alpha (TAGLN/247), CF647 Conjugate

Produktmerkmale: This MAb recognizes a 22 kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human transgelin (TAGLN) protein.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry 0.5-1 μg/million cells/0.1 mLImmunofluorescence 1-2 μg/mL

Kommentare:

U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Transgelin

Target: Transgelin (TAGLN)

Andere Bezeichnung: Transgelin / SM22-alpha

Molekulargewicht: 22 kDa

Gen-ID: 6876, 410977

UniProt: Q01995