s100a4 Antikörper (CF®488A)

Produktnummer ABIN6167383

Der Maus Monoklonal Anti-s100a4-Antikörper wurde für WB, IHC, IF und FACS validiert. Er ist geeignet, s100a4 in Proben von Human und Maus zu detektieren.

Kurzübersicht für s100a4 Antikörper (CF®488A) (ABIN6167383)

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Reaktivität: Human, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser s100a4 Antikörper ist konjugiert mit CF®488A

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: S100A4-1481

Produktdetails anti-s100a4 Antikörper (CF®488A)

Verwendungszweck: Mouse Monoclonal anti-S100A4 (S100A4/1481), CF488A Conjugate

Produktmerkmale: S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, ?6?4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human S100A4 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.25-0.5 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HeLa, A549 or A375 Cells. Placenta

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu s100a4

Target: s100a4 (S100A4) (S100 Calcium Binding Protein A4 (S100A4))

Andere Bezeichnung: S100A4

Molekulargewicht: 10-12 kDa

Gen-ID: 6275, 654444

UniProt: P26447

Pathways: S100 Proteine