Vimentin Antikörper

Produktnummer ABIN6166864

Der Maus Monoklonal anti-Vimentin Antikörper wird verwendet zum Nachweis von Vimentin in Proben von Human, Ratte, Maus, Schwein, Rind (Kuh), Cat, Kaninchen und Schaf. Er wurde validiert für IF, FACS und IHC (f).

Kurzübersicht für Vimentin Antikörper (ABIN6166864)

Target: Vimentin (VIM)

Reaktivität: Human, Ratte, Maus, Schwein, Rind (Kuh), Cat, Kaninchen, Schaf

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Vimentin Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: LN-6

Produktdetails anti-Vimentin Antikörper

Verwendungszweck: Mouse Monoclonal anti-Vimentin (LN-6), Purified, with BSA

Produktmerkmale: This MAb reacts with a 58 kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin and shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells, and endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin.Ab-2 does not react with leukocyte common antigen-positive tissues such as lymphomas and leukemias. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Human thymic nuclear extract

Isotyp: IgM

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Jurkat cells, Sarcomas, Melanomas

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Vimentin

Target: Vimentin (VIM)

Andere Bezeichnung: Vimentin

Hintergrund: VIM

Molekulargewicht: 57-60 kDa

Gen-ID: 7431, 455493

UniProt: P08670

Pathways: Caspase Kaskade in der Apoptose