PTH Antikörper (C-Term) (CF®488A)

Produktnummer ABIN6166585

Der Maus Monoklonal Anti-PTH-Antikörper wurde für IHC, IF und FACS validiert. Er ist geeignet, PTH in Proben von Human zu detektieren.

Kurzübersicht für PTH Antikörper (C-Term) (CF®488A) (ABIN6166585)

Target: PTH (Parathyroid Hormone (PTH))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PTH Antikörper ist konjugiert mit CF®488A

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 3H9 PTH-1175

Produktdetails anti-PTH Antikörper (CF®488A)

Bindungsspezifität: C-Term

Verwendungszweck: Mouse Monoclonal anti-PTH (Parathyroid Hormone) (N-&-C-Terminal) (3H9 + PTH/1175), CF488A Conjugate

Produktmerkmale: The epitope of this MAb maps in the C-terminus of PTH, a hormone produced by the parathyroid gland that regulates the concentration of calcium and phosphorus in extracellular fluid. This hormone elevates blood Ca2 levels by dissolving the salts in bone and preventing their renal excretion.It is produced in the parathyroid gland as an 84 amino acid single chain polypeptide. It can also be secreted as N-terminal truncated fragments or C-terminal fragments after intracellular degradation, as in case of hypercalcemia. Defects in this gene are a cause of familial isolated hypoparathyroidism (FIH), also called autosomal dominant hypoparathyroidism or autosomal dominant hypocalcemia. FIH is characterized by hypocalcemia and hyperphosphatemia due to inadequate secretion of parathyroid hormone. Symptoms are seizures, tetany and cramps. FIH exist both as autosomal dominant and recessive forms of hypoparathyroidism. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Synthetic peptide corresponding to amino acids 1 to 34 of PTH conjugated to a carrier (3H9), Recombinant fragment (84 amino acid residues from C-terminus) of human PTH protein

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Human parathyroid gland carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu PTH

Target: PTH (Parathyroid Hormone (PTH))

Andere Bezeichnung: PTH

Substanzklasse: Hormone

Hintergrund: HPTH, Parathormone, Parathyrin, Parathyroid hormone 1 (PTH1), Parathyroid hormone (PTH)

Molekulargewicht: 9 kDa

Gen-ID: 5741, 37045

UniProt: P01270

Pathways: cAMP Metabolic Process, Regulation of Carbohydrate Metabolic Process