Moesin Antikörper (Biotin)

Produktnummer ABIN6165718

Der Maus Monoklonal Anti-Moesin-Antikörper wurde für WB, IF, FACS und IHC (f) validiert. Er ist geeignet, Moesin in Proben von Human zu detektieren.

Kurzübersicht für Moesin Antikörper (Biotin) (ABIN6165718)

Target: Moesin (MSN)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Moesin Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: MSN492

Produktdetails anti-Moesin Antikörper (Biotin)

Verwendungszweck: Mouse Monoclonal anti-Moesin (MSN492), Biotin Conjugate

Produktmerkmale: This antibody recognizes 78 kDa moesin protein. Moesin, a member of the talin-4.1 superfamily, is a linking protein of the sub-membranous actin cytoskeleton. It is expressed in variable amounts in cells of different phenotypes such as macrophages, lymphocytes, fibroblastic, endothelial, epithelial, and neuronal cell lines but not in blood cells. The ERM proteins, ezrin, radixin, and moesin are involved in a variety of cellular functions, such as cell adhesion, migration, and the organization of cell surface structures, and are highly homologous, both in protein sequence and in functional activity, with merlin/schwannomin, a neurofibromatosis-2-associated tumor-suppressor protein. Cell lines of epithelial and mesothelial origin contain both moesin and radixin whereas cells of endothelial and lymphoid origin express moesin. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Moesin protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Does not react with rat, others not known

• Immunohistology formalin-fixed 0.5-1 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HT-29, CH3LC, or HUVEC cells. Uterus, Placenta, Tonsil, Melanoma, Testicular Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu Moesin

Target: Moesin (MSN)

Andere Bezeichnung: Moesin

Hintergrund: Membrane-organizing extension spike protein, Moesin/anaplastic lymphoma kinase fusion protein, MSN/ALK fusion

Molekulargewicht: 78 kDa

Gen-ID: 4478, 87752

UniProt: P26038

Pathways: Asymmetric Protein Localization