TIMP2 Antikörper (N-Term)

Produktnummer ABIN6165498

Dieses Maus Monoklonal-Antikörper erkennt spezifisch TIMP2 in WB, IHC, IF und FACS. Er zeigt eine Reaktivität gegenüber Human, Ratte, Maus, Rind (Kuh), Zebrafisch (Danio rerio), Kaninchen und Meerschweinchen.

Kurzübersicht für TIMP2 Antikörper (N-Term) (ABIN6165498)

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Zebrafisch (Danio rerio), Kaninchen, Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TIMP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 3A4

Produktdetails anti-TIMP2 Antikörper

Bindungsspezifität: AA 56-66, N-Term

Verwendungszweck: Mouse Monoclonal anti-TIMP2 (3A4), Purified, BSA-free

Produktmerkmale: This antibody recognizes a protein of 21 kDa, identified as tissue inhibitor of metalloproteinases-2 (TIMP-2). It is closely related to TIMP-1 and shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: A synthetic peptide from the N-terminus of human TIMP2 protein (aa56-66) (DSGNDIYGNPI)

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HeLa cells. Normal Placenta. Breast, Colon, Endometrial, Prostate or Ovarian Carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Konservierungsmittel: Azide free

Details zu TIMP2

Target: TIMP2 (Metalloproteinase Inhibitor 2 (TIMP2))

Andere Bezeichnung: TIMP2

Hintergrund: Metalloproteinase inhibitor 2, TIMP metallopeptidase inhibitor 2, TIMP-2, TIMP2, Tissue Inhibitor of Metalloproteinase 2

Molekulargewicht: 21 kDa

Gen-ID: 7077, 633514

UniProt: P16035

Pathways: cAMP Metabolic Process