CD44v6 Antikörper (CF®594)

Produktnummer ABIN6165447

Dieses Maus Monoklonal-Antikörper erkennt spezifisch in IHC, FACS und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CD44v6 Antikörper (CF®594) (ABIN6165447)

Target: CD44v6

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: CF®594

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: CD44v6-1246

Produktdetails

Verwendungszweck: Mouse Monoclonal anti-CD44v6 (CD44v6/1246), CF594 Conjugate

Produktmerkmale: This antibody recognizes an epitope encoded by exon v6 on the variant portion of human CD44. The CD44 Molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment corresponding to the v6 domain of human CD44 (exact sequence is proprietary)

Isotyp: IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Cervix or Tongue Squamous Cell Carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Antigendetails

Target: CD44v6

Molekulargewicht: 80-95 kDa

Gen-ID: 960, 502328

UniProt: P16070