MVP Antikörper (CF®488A)

Produktnummer ABIN6165331

Der Maus Monoklonal Anti-MVP-Antikörper wurde für FACS und IF validiert. Er ist geeignet, MVP in Proben von Human zu detektieren.

Kurzübersicht für MVP Antikörper (CF®488A) (ABIN6165331)

Target: MVP (Major Vault Protein (MVP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MVP Antikörper ist konjugiert mit CF®488A

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 1014

Produktdetails anti-MVP Antikörper (CF®488A)

Verwendungszweck: Mouse Monoclonal anti-Major Vault Protein (MVP) (1014), CF488A Conjugate

Produktmerkmale: This antibody recognizes a protein of 104 kDa-110 kDa, characterized as major vault protein (MVP). Vaults are large ribonucleoprotein particles (RNPs) present in all eukaryotic cells. They have a complex morphology, including several small molecules of RNA, but a single protein species. The MVP accounts for >70 % of their mass. Their shape is reminiscent of the nucleopore central plug. Treatment of cells with estradiol increases the amount of MVP in nuclear extract. The hormone-dependent interaction of vaults with ER is prevented in vitro by sodium molybdate. Antibodies to estrogen, progesterone and glucocorticoid receptors are able to co-immunoprecipitate the MVP. MVP is overexpressed in many neoplastic tissues and cell lines. Expression of MVP predicts a poor response to chemotherapy. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Proteins precipitated from human breast cancer MCF-7 cells

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunofluorescence 0.5-1 μg/mL

• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

MCF-7/HeLa, breast tumors

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu MVP

Target: MVP (Major Vault Protein (MVP))

Hintergrund: Lung resistance-related protein (LRP), Major Vault Protein, MVP, VAULT1

Molekulargewicht: 104-110 kDa

Gen-ID: 9961, 632177

UniProt: Q14764