JUP Antikörper (CF®594)

Produktnummer ABIN6164953

Der Maus Monoklonal Anti-JUP-Antikörper wurde für WB, IF, IHC und FACS validiert. Er ist geeignet, JUP in Proben von Human zu detektieren.

Kurzübersicht für JUP Antikörper (CF®594) (ABIN6164953)

Target: JUP (Junction Plakoglobin (JUP))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser JUP Antikörper ist konjugiert mit CF®594

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Klon: CTNG-1483

Produktdetails anti-JUP Antikörper (CF®594)

Verwendungszweck: Mouse Monoclonal anti-Catenin, gamma (CTNG/1483), CF594 Conjugate

Produktmerkmale: This antibody recognizes a protein of 80-87 kDa, identified as gamma-catenin. The catenins (?, ?, ? and ?) are ubiquitously expressed, cytoplasmic proteins that associate with E-cadherin at cellular junctions. Catenin/cadherin complexes play an important role in mediating cellular adhesion. ? T-catenin, also referred to as VR22, is a 895-amino acid protein that is most abundantly expressed in cardio-myocytes and in the peritubular myoid cells of the testis. ? T-catenin binds to ? E-catenin as well as to ?-catenin, and it functions to inhibit Wnt signaling. CTNNA3, the gene that encodes for ?-T-catenin, is located on chromosome 10, and mutations in this gene show a strong correlation to late-onset Alzheimer s disease (LOAD) as well as to dilated cardiomyopathy. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length gamma catenin protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min

• Immunohistology (formalin) 0.1-0.2 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

A431, T47D or MCF-7 cells. Skin

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu JUP

Target: JUP (Junction Plakoglobin (JUP))

Andere Bezeichnung: Catenin, gamma

Hintergrund: ARVD12, Catenin (cadherin-associated protein), gamma 80  kDa, Catenin gamma, CTNNG, Desmoplakin III, Desmoplakin-3, DP3, DPIII, Junction Plakoglobin, PDGB, PKGB

Molekulargewicht: 80-87 kDa

Gen-ID: 3728, 514174

UniProt: P14923

Pathways: Cell-Cell Junction Organization, Maintenance of Protein Location