Mitochondrial Marker Antikörper (CF®488A)

Produktnummer ABIN6164001

Produktdetails

Target: Mitochondrial Marker

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: CF®488A

Applikation: Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Western Blotting (WB), Flow Cytometry (FACS), Immunostaining (ISt)

Verwendungszweck: Mouse Monoclonal anti-Mitochondrial Marker (MTC719), CF488A Conjugate

Produktmerkmale: This MAb recognizes a 60 kDa antigen associated with the mitochondria in cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. It recognizes an antigen associated with the mitochondria in cells from a wide variety of animals, but not insects and bacteria. It can be used to stain the mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells and may be used to stain mitochondria of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Mitochondrial fraction of HeLa cells

Klon: MTC719

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Does not react with insects or bacteria

• Immunohistology formalin-fixed 0.5-1 μg/mL
• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.25-0.5 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HeLa or HepG2 cells. Hepatic carcinomas.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Antigendetails

Target: Mitochondrial Marker

Molekulargewicht: 60 kDa