GNRHR Antikörper (N-Term) (Biotin)

Produktnummer ABIN6163438

Der Maus Monoklonal Anti-GNRHR-Antikörper wurde für IF, FACS und IHC (f) validiert. Er ist geeignet, GNRHR in Proben von Human und Ratte zu detektieren.

Kurzübersicht für GNRHR Antikörper (N-Term) (Biotin) (ABIN6163438)

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GNRHR Antikörper ist konjugiert mit Biotin

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: F1G4

Produktdetails anti-GNRHR Antikörper (Biotin)

Bindungsspezifität: AA 1-29, N-Term

Verwendungszweck: Mouse Monoclonal anti-GnRH Receptor (LHRH Receptor) (F1G4), Biotin Conjugate

Produktmerkmale: This antibody recognizes an epitope on the extracellular domain of gonadotropin releasing hormone (GnRH) receptor or luteinizing hormone receptor (LHCGR). Lutropin (also designated luteinizing hormone) plays a role in spermatogenesis and ovulation by stimulating the testes and ovaries to produce steroids. Gonadotropin (also designated choriogonadotropin) production in the placenta maintains estrogen and progesterone levels during the first trimester of pregnancy. Ovaries and testes abundantly express luteinizing hormone/choriogonadotropin receptor. GnRH receptor contains seven hydrophobic transmembrane domains connected by hydrophilic extracellular and intracellular loops characteristic of G-protein coupled receptors. GnRH stimulates the gonadotrophs of the anterior pituitary to secrete luteinizing hormone (LH) as well as follicle-stimulating hormone (FSH). GnRH influences the protective effect of pregnancy and Gonadotropin against breast cancer. The expression of GnRH on breast carcinoma correlates in part to the degree of tumor differentiation. GnRH-positive breast tumors occur more frequently in tumors with greater cell differentiation in premenopausal women. GnRH is present in luteal and granulosa cells as well as in ovarian cell membrane preparations. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: A synthetic peptide aa 1-29 (MANSASPEQNQHCSAINNSIPLMQGNLPY) from the N-terminal of human GnRH receptor.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Predicted to react with pig or rabbit, others not known
• Optimal dilution for a specific application should be determined by user

Kommentare:

T47D cells. Pituitary gland, ovarian or breast cancers.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu GNRHR

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Andere Bezeichnung: GnRH Receptor (LHRH Receptor)

Molekulargewicht: 54-60 kDa