Rekombinanter Caldesmon HMW Antikörper (Biotin)

Produktnummer ABIN6162488

Produktdetails

Target: Caldesmon HMW

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Biotin

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Verwendungszweck: Recombinant Rabbit Monoclonal anti-Caldesmon, HMW (CALD1/1424R), Biotin Conjugate

Produktmerkmale: This antibody recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length CALD1 protein

Klon: CALD1-1424R

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 1-2 μg/mL

• for 10-20 min followed by cooling at RT for 20 min
• Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA
•   pH 7.5-8.5
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Antigendetails

Target: Caldesmon HMW

Andere Bezeichnung: Caldesmon, HMW

Hintergrund: CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22

Molekulargewicht: 150 kDa

Gen-ID: 800, 490203

UniProt: Q05682