Desmoglein 3 Antikörper (CF®594)

Produktnummer ABIN6162293

Dieses Anti-Desmoglein 3-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Desmoglein 3 in WB, IHC, IF und FACS. Geeignet für Human.

Kurzübersicht für Desmoglein 3 Antikörper (CF®594) (ABIN6162293)

Target: Desmoglein 3 (DSG3)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmoglein 3 Antikörper ist konjugiert mit CF®594

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 5G11

Produktdetails anti-Desmoglein 3 Antikörper (CF®594)

Verwendungszweck: Mouse Monoclonal anti-Desmoglein-3 (5G11), CF594 Conjugate

Produktmerkmale: This antibody recognizes a protein of 130 kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human DSG3 extracellular protein fragment (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

A431 cells. Lung squamous cell carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Desmoglein 3

Target: Desmoglein 3 (DSG3)

Andere Bezeichnung: Desmoglein-3

Hintergrund: 130  kDa pemphigus vulgaris antigen (PVA), Balding (Bal), Cadherin family member 6 (CDHF6), Desmoglein-3 (DSG3)

Molekulargewicht: 130 kDa

Gen-ID: 1830, 1925

UniProt: P32926