Desmoglein 1 Antikörper (CF®488A)

Produktnummer ABIN6162139

Der Maus Monoklonal Anti-Desmoglein 1-Antikörper wurde für IHC, IF und FACS validiert. Er ist geeignet, Desmoglein 1 in Proben von Human zu detektieren.

Kurzübersicht für Desmoglein 1 Antikörper (CF®488A) (ABIN6162139)

Target: Desmoglein 1 (DSG1)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmoglein 1 Antikörper ist konjugiert mit CF®488A

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: DSG1-1733

Produktdetails anti-Desmoglein 1 Antikörper (CF®488A)

Verwendungszweck: Mouse Monoclonal anti-Desmoglein-1 (DSG1/1733), CF488A Conjugate

Produktmerkmale: This antibody recognizes a protein of ~150 kDa, identified as Desmoglein-1 (DSG1). Desmoglein-1 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human desmoglein-1 protein fragment corresponding to intracellular domain (exact sequence is proprietary)

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA  pH 8.0 for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

A431 or A-375 cells. Skin

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Desmoglein 1

Target: Desmoglein 1 (DSG1)

Andere Bezeichnung: Desmoglein-1

Hintergrund: Cadherin family member 4, CDHF4, Desmoglein-1, Desmosomal glycoprotein 1, DG1, DSG1, EPKHE, EPKHIA, Pemphigus foliaceus antigen, PPKS1, SPPK1

Molekulargewicht: 150 kDa (precursor), 160 kDa (mature)

Gen-ID: 1828, 2633

UniProt: Q02413

Pathways: Cell-Cell Junction Organization