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KRT1 Antikörper (CF®647)

KRT1 Reaktivität: Human, Ratte, Maus, Kaninchen, Huhn, Rind (Kuh), Affe, Hund WB, IF, FACS, IHC (f) Wirt: Maus Monoclonal AE-3 CF®647
Produktnummer ABIN6161973
  • Target Alle KRT1 Antikörper anzeigen
    KRT1 (Cytokeratin HMW (KRT1))
    Reaktivität
    • 27
    • 27
    • 18
    • 9
    • 9
    • 9
    • 9
    • 8
    • 1
    Human, Ratte, Maus, Kaninchen, Huhn, Rind (Kuh), Affe, Hund
    Wirt
    • 27
    Maus
    Klonalität
    • 27
    Monoklonal
    Konjugat
    • 7
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    Dieser KRT1 Antikörper ist konjugiert mit CF®647
    Applikation
    • 26
    • 26
    • 17
    • 15
    • 9
    • 9
    • 2
    • 2
    Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    Verwendungszweck
    Mouse Monoclonal anti-Cytokeratin, HMW (AE-3), CF647 Conjugate
    Produktmerkmale
    This MAb recognizes basic (Type II or HMW) cytokeratins, which include 67 kDa (CK1), 64 kDa (CK3), 59 kDa (CK4), 58 kDa (CK5), 56 kDa (CK6), 52 kDa (CK8). Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. The acidic keratins have molecular weights (MW) of 56.5, 55, 51, 50, 50 , 48, 46, 45, and 40 kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52 kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail, which differentiates epithelial tumors from non-epithelial tumors e.g. squamous vs. adenocarcinoma of the lung, liver carcinoma, breast cancer, and esophageal cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
    Immunogen
    Human epidermal keratin
    Klon
    AE-3
    Isotyp
    IgG1 kappa
    Top Product
    Discover our top product KRT1 Primärantikörper
  • Applikationshinweise
    Immunohistology formalin-fixed 0.25-0.5 μg/mL
    • Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    • Flow Cytometry 0.5-1 μg/million cells/0.1 mL
    • Immunofluorescence 1-2 μg/mL
    • Western blotting 0.5-1 μg/mL
    • Optimal dilution for a specific application should be determined by user
    Kommentare

    Epithelial cells, Skin or Adenocarcinomas

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    100 μg/mL
    Buffer
    PBS/0.1 % BSA/0.05 % azide
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handhabung
    Protect from light
  • Target
    KRT1 (Cytokeratin HMW (KRT1))
    Andere Bezeichnung
    Cytokeratin, HMW (KRT1 Produkte)
    Synonyme
    CK1 antikoerper, EHK antikoerper, EHK1 antikoerper, EPPK antikoerper, K1 antikoerper, KRT1A antikoerper, NEPPK antikoerper, keratin 1 antikoerper, KRT1 antikoerper
    Hintergrund
    KRT2B, KRT2P, HUMCYT2A, Keratin, type II Cytoskeletal 2 oral, K76, Keratin 2p (K2P), Keratin-76, Cytokeratin-2P (CK-2P, Type-II Keratin Kb9
    Molekulargewicht
    52-67 kDa
    Gen-ID
    51350, 654392
    UniProt
    Q01546
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