VWF Antikörper (AA 845-949) (Biotin)
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- Target Alle VWF Antikörper anzeigen
- VWF (Von Willebrand Factor (VWF))
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Bindungsspezifität
- AA 845-949
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Reaktivität
- Human
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Wirt
- Maus
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Klonalität
- Monoklonal
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Konjugat
- Dieser VWF Antikörper ist konjugiert mit Biotin
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF)
- Verwendungszweck
- Mouse Monoclonal anti-von Willebrand Factor (3E2D10), Biotin Conjugate
- Produktmerkmale
- von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
- Immunogen
- Recombinant human vWF fragment spanning aa 845-949
- Klon
- 3E2D10
- Isotyp
- IgG1 kappa
- Top Product
- Discover our top product VWF Primärantikörper
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- Applikationshinweise
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Immunohistology (formalin)
- Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
- Immunofluorescence 0.5-1 μg/mL
- Flow Cytometry 0.5-1 μg/million cells/0.1 mL
- Western blotting 0.5-1.0 μg/mL
- Immunoprecipitation 0.5-1 μg/500 μg protein lysate
- Optimal dilution for a specific application should be determined by user
- Kommentare
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HUVEC cells or Tonsil
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Liquid
- Konzentration
- 100 μg/mL
- Buffer
- PBS/0.1 % BSA/0.05 % azide
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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- Target
- VWF (Von Willebrand Factor (VWF))
- Andere Bezeichnung
- von Willebrand Factor (VWF Produkte)
- Synonyme
- VWF antikoerper, si:ch1073-474e24.1 antikoerper, F8VWF antikoerper, VWD antikoerper, 6820430P06Rik antikoerper, AI551257 antikoerper, B130011O06Rik antikoerper, C630030D09 antikoerper, von Willebrand factor antikoerper, Von Willebrand factor antikoerper, VWF antikoerper, vwf antikoerper, Vwf antikoerper
- Hintergrund
- Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)
- Molekulargewicht
- 250 kDa
- Gen-ID
- 7450, 440848
- UniProt
- P04275
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