VWF Antikörper

Produktnummer ABIN6161506

Der Maus Monoklonal Anti-VWF-Antikörper wurde für WB, IHC, IP, FACS und IF validiert. Er ist geeignet, VWF in Proben von Human zu detektieren.

Kurzübersicht für VWF Antikörper (ABIN6161506)

Target: VWF (Von Willebrand Factor (VWF))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VWF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: 3E2D10 VWF635

Produktdetails anti-VWF Antikörper

Verwendungszweck: Mouse Monoclonal anti-von Willebrand Factor (3E2D10 + VWF635), Purified, with BSA

Produktmerkmale: von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi's sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Recombinant human vWF fragment

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Western blotting 0.5-1.0 μg/mL
• Immunoprecipitation 0.5-1 μg/500 μg protein lysate
• Optimal dilution for a specific application should be determined by user

Kommentare:

HUVEC cells or Tonsil

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 200 μg/mL

Buffer: PBS/0.05 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu VWF

Target: VWF (Von Willebrand Factor (VWF))

Andere Bezeichnung: von Willebrand Factor

Hintergrund: Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebrand Antigen 2, von Willebrand Disease (vWD)

Molekulargewicht: 250 kDa

Gen-ID: 7450, 440848

UniProt: P04275