Rekombinanter CD99 Antikörper (CF®568)

Produktnummer ABIN6160421

Dieses Anti-CD99-Antikörper ist ein Kaninchen Monoklonal-Antikörper zur Detektion von CD99 in FACS, IHC und IF. Geeignet für Human.

Kurzübersicht für Rekombinanter CD99 Antikörper (CF®568) (ABIN6160421)

Target: CD99

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD99 Antikörper ist konjugiert mit CF®568

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: MIC2-1495R

Produktdetails anti-CD99 Antikörper (CF®568)

Verwendungszweck: Recombinant Rabbit Monoclonal anti-CD99 / MIC2 (MIC2/1495R), CF568 Conjugate

Produktmerkmale: This antibody recognizes a sialoglycoprotein of 27-32 kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32 kDa (p30/32). Although its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis. CD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length MIC2 protein

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Flow cytometry 2-4 μg/million cells

• Immunofluorescence 2-4 μg/mL
• Immunohistology (formalin) 5-10 μg/mL
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Optimal dilution for a specific application should be determined by user

Kommentare:

MOLT-4 cells. Pancreas or Ewing s sarcoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu CD99

Target: CD99

Andere Bezeichnung: CD99 / MIC2

Hintergrund: 12E7, E2 antigen, MIC 2X, MIC 2Y, MIC2, Protein MIC2, Surface antigen MIC2, T-cell surface glycoprotein E2

Molekulargewicht: 27-32 kDa

Gen-ID: 4267, 653349

UniProt: P14209