ESAM Antikörper (AA 30-248)

Produktnummer ABIN616014

Der Kaninchen Polyklonal Anti-ESAM-Antikörper wurde für WB und IF validiert. Er ist geeignet, ESAM in Proben von Human zu detektieren.

Kurzübersicht für ESAM Antikörper (AA 30-248) (ABIN616014)

Target: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ESAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Produktdetails anti-ESAM Antikörper

Bindungsspezifität: AA 30-248

Spezifität: This antibody will detect recombinant human soluble ESAM in Western blot and native ESAM in Immunoflourescence.

Kreuzreaktivität (Details): Species reactivity (tested):Human

Aufreinigung: Protein A affinity chromatography

Immunogen: Highly pure (>95%) recombinant human ESAM (Ile30-Ala248) derived from insect cells.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore in sterile water to a concentration of 1.0 mg/mL.

Buffer: PBS pH 7.4 w/o preservative

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: The lyophilized antibody is stable at room temperature for up to 1 month. Following reconstitution antibody can be stored at 2-8 °C for up to two weeks or (in aliquots) at -20 °C for longer.

Details zu ESAM

Target: ESAM (Endothelial Cell Adhesion Molecule (ESAM))

Andere Bezeichnung: ESAM

Hintergrund: Endothelial cellselective adhesion molecule (ESAM) is a 55 kDa type I transmembrane glycoprotein that belongs to the JAM family of immunoglobulin superfamily molecules. Human ESAM is synthesized as a 390 amino acid (aa) protein composed of a 29 aa signal peptide, a 216 aa extracellular region, a putative 26 aa transmembrane segment, and a 119 aa cytoplasmic domain. The extracellular region contains one V-type and one C2-type Ig domain and is involved in hemophilic adhesion. In the cytoplasmic domain, there is a docking site for the multifunctional adaptor protein MAGI1. The extracellular region of human ESAM shows 90 % , 74 %, 69 % and 67 % aa identity with monkey, canine, mouse and rat extracellular ESAM, respectively. ESAM is expressed on endothelial cells, activated platelets and megakaryocytes, and can be found associated with cell to cell junctions. Whether ESAM is restricted to a particular junctional type is not clear. ESAM deficient mice have no defect in vascularization but do have reduced angiogenic potential. This may be due to a decreased migratory response to FGF2. Soluble ESAM is fused to a C-terminal His-tag (6x His).Synonyms: Endothelial cell-selective adhesion molecule

Gen-ID: 90952

NCBI Accession: NP_620411

UniProt: Q96AP7