EpCAM Antikörper (Extracellular Domain) (CF®594)

Produktnummer ABIN6158987

Produktdetails anti-EpCAM Antikörper (CF®594)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Bindungsspezifität: AA 77-202, Extracellular Domain

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist konjugiert mit CF®594

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Coating (Coat)

Verwendungszweck: Mouse Monoclonal anti-Ep-CAM / CD326 (EGP40/1798), CF594 Conjugate

Produktmerkmale: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human EpCAM protein fragment from the extracellular domain (aa77-202) (exact sequence is proprietary)

Klon: EGP40-1798

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: ELISA 2-4 μg/mL for coating (order Ab without BSA)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HT29 cells or Ovarian carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: Ep-CAM / CD326 (EPCAM Produkte)

Molekulargewicht: 40-43 kDa

Gen-ID: 4072, 542050

UniProt: P16422