EpCAM Antikörper (Extracellular Domain) (Biotin)

Produktnummer ABIN6158878

Der Maus Monoklonal Anti-EpCAM Antikörper wurde für WB, FACS, IHC und IF validiert. Er ist geeignet, EpCAM in Proben von Human zu detektieren.

Kurzübersicht für EpCAM Antikörper (Extracellular Domain) (Biotin) (ABIN6158878)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser EpCAM Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: EGP40-1372

Produktdetails anti-EpCAM Antikörper (Biotin)

Bindungsspezifität: AA 77-202, Extracellular Domain

Verwendungszweck: Mouse Monoclonal anti-Ep-CAM / CD326 (EGP40/1372), Biotin Conjugate

Produktmerkmale: EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human EpCAM protein fragment from the extracellular domain (aa77-202) (exact sequence is proprietary)

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HT29 cells or Ovarian carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: Ep-CAM / CD326

Molekulargewicht: 40-43 kDa

Gen-ID: 4072, 542050

UniProt: P16422