SELPLG Antikörper (CF®568)

Produktnummer ABIN6157951

Der Maus Monoklonal Anti-SELPLG-Antikörper wurde für FACS, IHC, IF und ISt validiert. Er ist geeignet, SELPLG in Proben von Human zu detektieren.

Kurzübersicht für SELPLG Antikörper (CF®568) (ABIN6157951)

Target: SELPLG (Selectin P Ligand (SELPLG))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser SELPLG Antikörper ist konjugiert mit CF®568

Applikation: Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunostaining (ISt)

Klon: PSGL1-1601

Produktdetails anti-SELPLG Antikörper (CF®568)

Verwendungszweck: Mouse Monoclonal anti-CD162 (PSGL1/1601), CF568 Conjugate

Produktmerkmale: CD162 glycoprotein functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human full-length SELPLG (CD162) protein

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 1-2 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM Tris, 1 mM EDTA  pH 9.0 for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Jurkat cells. Tonsil or Spleen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu SELPLG

Target: SELPLG (Selectin P Ligand (SELPLG))

Andere Bezeichnung: CD162

Hintergrund: CD162, CLA, Cutaneous lymphocyte associated antigen, P-selectin glycoprotein lig, 1, PSGL1, Selectin P lig, SELPLG

Molekulargewicht: 43-120 kDa

Gen-ID: 6404, 591014

UniProt: Q14242