Melanoma Marker Antikörper (CF®594)

Produktnummer ABIN6157201

Der Maus Monoklonal Anti-Melanoma Marker-Antikörper wurde für IHC und IF validiert. Er ist geeignet, Melanoma Marker in Proben von Human, Hund und Maus zu detektieren.

Kurzübersicht für Melanoma Marker Antikörper (CF®594) (ABIN6157201)

Target: Melanoma Marker

Reaktivität: Human, Hund, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Melanoma Marker Antikörper ist konjugiert mit CF®594

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF)

Klon: PNL2

Produktdetails anti-Melanoma Marker Antikörper (CF®594)

Verwendungszweck: Mouse Monoclonal anti-Melanoma Marker (PNL2), CF594 Conjugate

Produktmerkmale: Anti-PNL2 is a novel monoclonal antibody, which has recently been introduced as an immunohistochemical reagent to stain melanocytes and tumors derived therefrom. The antigen recognized by PNL2 is different from Melan A and gp100. Its epitope is not destroyed by digestion with neuraminidase i.e. its epitope id not glycosylated. Anti-PNL2 may be most useful because of its high sensitivity for metastatic melanoma (87 % ), as opposed to 76 % for anti-HMB45 and 82 % for anti-MART-1. Anti-PNL2 labels intra-epidermal nevi while the dermal component of compound nevi are largely non-reactive with anti-PNL2. Antibodies against PNL2, MART-1 (Melan A) and HMB45 stain most clear cell sarcoma cells and a few cells in angio-myolipomas and lymphangioleiomyomatosis. Anti-PNL2 is a useful antibody for the identification of melanomas and clear cell sarcomas. Differential diagnosis is aided by the results from a panel of antibodies, including antibodies against HMB45, MART-1, tyrosinase, and MiTF. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. See the < a href="https://biotium.com/wp-content/uploads/2013/07/CF-dye-selection-guide.pdf" target="_blank" rel="noopener">CF® Dye Selection Guide for more information. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Melanocyte antigen

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Melanoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Melanoma Marker

Target: Melanoma Marker

Hintergrund: Human Melanoma Associated Antigen, PNL2

Molekulargewicht: Multiple (160 kDa, 100 kDa and <10 kDa)