Melanoma Marker Antikörper (CF®405S)

Produktnummer ABIN6157101

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Melanoma Marker in IHC (f) und ISt. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Melanoma Marker Antikörper (CF®405S) (ABIN6157101)

Target: Melanoma Marker

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Melanoma Marker Antikörper ist konjugiert mit CF®405S

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)

Klon: HMB45 M2-7C10 M2-9E3 T311

Produktdetails anti-Melanoma Marker Antikörper (CF®405S)

Verwendungszweck: Mouse Monoclonal anti-Melanoma Marker (HMB45 + M2-7C10 + M2-9E3 + T311), CF405S Conjugate

Produktmerkmale: This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant hMART-1 protein (M2-7C10, M2-9E3), Recombinant tyrosinase protein (T311), Extract of pigmented melanoma metastases from lymph nodes (HMB45)

Isotyp: IgG1, IgG2a, IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (Formalin-fixed) 0.5-1.0 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Optimal dilution for a specific application should be determined by user

Kommentare:

SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Melanoma Marker

Target: Melanoma Marker

Hintergrund: Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A, TYR, PMEL17

Molekulargewicht: 20-22 kDa (doublet) (MART), 70-80 kDa (Tyrosinase), 90-100 kDa (gp100)