Melanoma Marker Antikörper

Produktnummer ABIN6157100

Der Maus Monoklonal anti-Melanoma Marker Antikörper (Klon HMB45 A103 T311) (ABIN6157100) detektiert spezifisch Melanoma Marker in IHC (f) und ISt. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Melanoma Marker Antikörper (ABIN6157100)

Target: Melanoma Marker

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Melanoma Marker Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunostaining (ISt)

Klon: HMB45 A103 T311

Produktdetails anti-Melanoma Marker Antikörper

Verwendungszweck: Mouse Monoclonal anti-Melanoma Marker (HMB45 + A103 + T311), Purified, BSA-free

Produktmerkmale: This antibody cocktail recognizes three melanoma-specific proteins, which include MART-1, Tyrosinase and gp100. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. The function of gp100 is not known but it is reported to be a useful marker for melanocytes and melanomas. This cocktail of three markers is designed for extremely sensitive labeling of formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Aufreinigung: Purified

Immunogen: Recombinant hMART-1 protein (A103), Recombinant tyrosinase protein (T311), Extract of pigmented melanoma metastases from lymph nodes (HMB45)

Isotyp: IgG1, IgG2a kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (Formalin-fixed) 0.5-1.0 μg/mL

• Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Optimal dilution for a specific application should be determined by user

Kommentare:

SK-MEL-13 and SK-MEL-19 Melanoma cell lines, Melanomas

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: PBS (no BSA, no azide)

Konservierungsmittel: Azide free

Details zu Melanoma Marker

Target: Melanoma Marker

Hintergrund: Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A, TYR, PMEL17

Molekulargewicht: 20-22 kDa (doublet) (MART), 70-80 kDa (Tyrosinase), 90-100 kDa (gp100)