Rekombinanter Histone H1 Antikörper (CF®594)

Produktnummer ABIN6156973

Der Kaninchen Monoklonal Anti-Histone H1-Antikörper wurde für IF, IHC und FACS validiert. Er ist geeignet, Histone H1 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für Rekombinanter Histone H1 Antikörper (CF®594) (ABIN6156973)

Target: Histone H1

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser Histone H1 Antikörper ist konjugiert mit CF®594

Applikation: Immunofluorescence (IF), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Klon: HH1-1784R

Produktdetails anti-Histone H1 Antikörper (CF®594)

Verwendungszweck: Recombinant Rabbit Monoclonal anti-Histone H1 (HH1/1784R), CF594 Conjugate

Produktmerkmale: Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer, formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80 % of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant full-length human Histone H1 protein

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Histone H1

Target: Histone H1

Molekulargewicht: ~30 kDa

Gen-ID: 3005