Alkaline Phosphatase Antikörper (CF®647)

Produktnummer ABIN6155855

Dieses Anti-Alkaline Phosphatase-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Alkaline Phosphatase in IF, FACS und IHC (f). Geeignet für Human und Rind (Kuh).

Kurzübersicht für Alkaline Phosphatase Antikörper (CF®647) (ABIN6155855)

Target: Alkaline Phosphatase (ALP)

Reaktivität: Human, Rind (Kuh)

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Alkaline Phosphatase Antikörper ist konjugiert mit CF®647

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: ALPL-597

Produktdetails anti-Alkaline Phosphatase Antikörper (CF®647)

Verwendungszweck: Mouse Monoclonal anti-Alkaline Phosphatase (ALPL/597), CF647 Conjugate

Produktmerkmale: This antibody reacts with tissue non-specific alkaline phosphatase. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization, however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene. Primary antibodies are available purified, or with a selection of fluorescent CF ® dyes and other labels. CF ® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF ®405S and CF ®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human ALP1 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Flow Cytometry 5-10 uL/million cells in 0.1 mL

• Immunohistology formalin-fixed 1:50-1:100
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 1:50-1:100
• Optimal dilution for a specific application should be determined by user

Kommentare:

Intestine

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu Alkaline Phosphatase

Target: Alkaline Phosphatase (ALP)

Andere Bezeichnung: Alkaline Phosphatase

Molekulargewicht: 55 kDa

Gen-ID: 248, 284255, 333509

UniProt: P05186, P05187