VWF Antikörper (AA 1815-1939) (CF®488A)

Produktnummer ABIN6154653

Dieses Maus Monoklonal-Antikörper erkennt spezifisch VWF in IHC, FACS, IF, ELISA und Coat. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für VWF Antikörper (AA 1815-1939) (CF®488A) (ABIN6154653)

Target: VWF (Von Willebrand Factor (VWF))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VWF Antikörper ist konjugiert mit CF®488A

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF), ELISA, Coating (Coat)

Klon: VWF-1767

Produktdetails anti-VWF Antikörper (CF®488A)

Bindungsspezifität: AA 1815-1939

Verwendungszweck: Mouse Monoclonal anti-von-Willebrand Factor / Factor-VIII-Related Ag (VWF/1767), CF488A Conjugate

Produktmerkmale: von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)

Isotyp: IgG2b kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 1-2 μg/mL

• ELISA 2-4 μg/mL for coating (order Ab without BSA)
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HUVEC cells or Tonsil

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu VWF

Target: VWF (Von Willebrand Factor (VWF))

Andere Bezeichnung: von-Willebrand Factor / Factor-VIII-Related Ag

Hintergrund: Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebr, Antigen 2, von Willebr, Disease (vWD)

Molekulargewicht: 250 kDa

Gen-ID: 7450, 440848

UniProt: P04275