VWF Antikörper (AA 1815-1939) (CF®405S)

Produktnummer ABIN6154593

Produktdetails anti-VWF Antikörper (CF®405S)

Target: VWF (Von Willebrand Factor (VWF))

Bindungsspezifität: AA 1815-1939

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser VWF Antikörper ist konjugiert mit CF®405S

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunofluorescence (IF)

Verwendungszweck: Mouse Monoclonal anti-von-Willebrand Factor / Factor-VIII-Related Ag (VWF/1465), CF405S Conjugate

Produktmerkmale: von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)

Klon: VWF-1465

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 1-2 μg/mL

• Western blotting 0.5-1 μg/mL Immunoprecipitation 0.5-1 μg/500 μg protein lysate
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HUVEC cells or Tonsil

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu VWF

Target: VWF (Von Willebrand Factor (VWF))

Andere Bezeichnung: von-Willebrand Factor / Factor-VIII-Related Ag (VWF Produkte)

Hintergrund: Coagulation Factor VIII, Factor VIII Related Antigen, F8VWF, von Willebr, Antigen 2, von Willebr, Disease (vWD)

Molekulargewicht: 250 kDa

Gen-ID: 7450, 440848

UniProt: P04275