Blood Group Lewis Y Antikörper (CF®640R)

Produktnummer ABIN6153115

Produktdetails

Target: Blood Group Lewis Y

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: CF®640R

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)

Verwendungszweck: Mouse Monoclonal anti-Lewis-Y (A70-A/A9), CF640R Conjugate

Produktmerkmale: This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis Y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta). Lewis Y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis Y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Live Ls174T cells (human colon carcinoma cell line)

Klon: A70-A-A9

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin) 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Lung, Colon, Bladder or Hepatocellular Carcinoma

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Antigendetails

Target: Blood Group Lewis Y

Andere Bezeichnung: Lewis-Y

Hintergrund: Lewis Y antigen

Molekulargewicht: Multiple