LGALS1/Galectin 1 Antikörper (AA 12-108) (CF®647)

Produktnummer ABIN6152663

Dieses Anti-LGALS1/Galectin 1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von LGALS1/Galectin 1 in WB, IHC, ELISA, FACS, IF und Coat. Geeignet für Human.

Kurzübersicht für LGALS1/Galectin 1 Antikörper (AA 12-108) (CF®647) (ABIN6152663)

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser LGALS1/Galectin 1 Antikörper ist konjugiert mit CF®647

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Coating (Coat)

Klon: GAL1-1831

Produktdetails anti-LGALS1/Galectin 1 Antikörper (CF®647)

Bindungsspezifität: AA 12-108

Verwendungszweck: Mouse Monoclonal anti-Galectin-1 / Human Placental Lactogen (GAL1/1831), CF647 Conjugate

Produktmerkmale: Galectin-1 is a member of the beta-galactoside-binding family and is a dimeric protein of 14 kD participating in a variety of normal and pathological processes, including cancer progression. Galectin-1 can affect the proliferation of normal and malignant cells. Inhibition of cell growth is observed in a lactose-dependent manner as lower concentrations of the lectin stimulate cell proliferation. Galectin-1 may also be implicated in the induction of apoptosis of activated T cells through the binding of exogenous galectin-1 to CD45 Molecules present on the surface of lymphocytes. Galectin-1, reported to be present either at the surface of cancer cells or accumulated around these cells could act as an immunological shield to protect against a T cell immune response and provide an advantage for survival. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human Galectin-1 protein fragment (aa12-108) (exact sequence is proprietary)

Isotyp: IgG Mix lambda

Anwendungsinformationen

Applikationshinweise: Flow cytometry 0.5-1 μg/million cells in 0.1 mL

• Immunohistology (formalin) 0.5-1 μg/mL
• ELISA 2-4 μg/mL for coating (order Ab without BSA)
• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
• Immunofluorescence 1-2 μg/mL
• Western blotting 0.5-1 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

HeLa, K562 or 293 cells. Prostate, Kidney, Placenta, Stomach, Skin, Spleen, Brain or Heart.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu LGALS1/Galectin 1

Target: LGALS1/Galectin 1 (LGALS1) (Lectin, Galactoside-Binding, Soluble, 1 (LGALS1))

Andere Bezeichnung: Galectin-1 / Placental Lactogen

Molekulargewicht: 14 kDa

Gen-ID: 3956, 445351

UniProt: P09382

Pathways: Carbohydrate Homeostasis