S100A8/A9 Complex (Calprotectin) Antikörper (CF®594)

Produktnummer ABIN6152299

Der Maus Monoklonal Anti-S100A8/A9 Complex (Calprotectin)-Antikörper wurde für FACS, IF und IHC (f) validiert. Er ist geeignet, S100A8/A9 Complex (Calprotectin) in Proben von Human, Schwein, Rind (Kuh), Cat, Hund, Meerschweinchen, Affe, Kaninchen, Ratte, Maus, Pferd, Pavian und Ziege zu detektieren.

Kurzübersicht für S100A8/A9 Complex (Calprotectin) Antikörper (CF®594) (ABIN6152299)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Reaktivität: Human, Schwein, Rind (Kuh), Cat, Hund, Meerschweinchen, Affe, Kaninchen, Ratte, Maus, Pferd, Pavian, Ziege

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser S100A8/A9 Complex (Calprotectin) Antikörper ist konjugiert mit CF®594

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Klon: MAC387

Produktdetails anti-S100A8/A9 Complex (Calprotectin) Antikörper (CF®594)

Verwendungszweck: Mouse Monoclonal anti-Calgranulin-B / MRP-8/-14 / MAC-387 / S100A8/A9 / Macrophage Ag (MAC387), CF594 Conjugate

Produktmerkmale: This antibody recognizes the L1 or Calprotectin molecule, an intra-cytoplasmic antigen comprising of a 12 kDa alpha chain and a 14 kDa beta chain expressed by granulocytes, monocytes and by tissue macrophages. Macrophages usually arise from hematopoietic stem cells in the bone marrow. Under migration into tissues, the monocytes undergo further differentiation to become multifunctional tissue macrophages. They are classified into normal and inflammatory macrophages. Normal macrophages include macrophages in connective tissue (histiocytes), liver (Kupffer's cells), lung (alveolar macrophages), lymph nodes (free and fixed macrophages), spleen (free and fixed macrophages), bone marrow (fixed macrophages), serous fluids (pleural and peritoneal macrophages), skin (histiocytes, Langerhans's cell) and in other tissues. Inflammatory macrophages are present in various exudates. Macrophages are part of the innate immune system, recognizing, engulfing and destroying many potential pathogens including bacteria, pathogenic protozoa, fungi and helminthes. This MAb reacts with neutrophils, monocytes, macrophages, and squamous mucosal epithelia and has been shown as an important marker for identifying macrophages in tissue sections. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Affinity purified monocyte membrane preparation

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology formalin-fixed 0.5-1 μg/mL

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
• Immunofluorescence 0.5-1 μg/mL
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

Tonsil, lymph node, or spleen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu S100A8/A9 Complex (Calprotectin)

Target: S100A8/A9 Complex (Calprotectin) (S100A8/A9) (Calprotectin (S100A8/A9))

Andere Bezeichnung: Calgranulin-B / MRP-8/-14 / MAC-387 / S100A8/A9 / Macrophage Ag

Molekulargewicht: 12-14 kDa (doublet)

Gen-ID: 6279, 6280

Pathways: S100 Proteine