MCAM Antikörper (CF®594)

Produktnummer ABIN6151311

Dieses Maus Monoklonal-Antikörper erkennt spezifisch MCAM in IHC, FACS und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für MCAM Antikörper (CF®594) (ABIN6151311)

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MCAM Antikörper ist konjugiert mit CF®594

Applikation: Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Klon: C146-634

Produktdetails anti-MCAM Antikörper (CF®594)

Verwendungszweck: Mouse Monoclonal anti-CD146 / MCAM / MUC18 / Mucin 18 (C146/634), CF594 Conjugate

Produktmerkmale: The human CD146 gene maps to chromosome 11q23 and encodes a trans-membrane glycoprotein, also designated MCAM, MUC 18, that belongs to the immunoglobulin superfamily and functions as a Ca2 -independent cell adhesion molecule. CD146 expression is restricted to advanced primary and metastatic melanomas and to cell lines of the neuroectodermal lineage, but not normal melanocytes. CD146 is found on 80 % of advanced primary human melanomas and correlates well with development of metastatic disease. Primary antibodies are available purified, or with a selection of fluorescent CF® dyes and other labels. CF® dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.

Immunogen: Recombinant human CD146 protein

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Immunohistology (formalin)

• Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA,  pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes
• Flow Cytometry 0.5-1 μg/million cells/0.1 mL
• Immunofluorescence 0.5-1.0 μg/mL
• Optimal dilution for a specific application should be determined by user

Kommentare:

A-375, HUV-EC or HeLa Cells. Tonsil or Melanoma.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 100 μg/mL

Buffer: PBS/0.1 % BSA/0.05 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Protect from light

Details zu MCAM

Target: MCAM (Melanoma Cell Adhesion Molecule (MCAM))

Andere Bezeichnung: CD146 / MCAM / MUC18 / Mucin 18

Molekulargewicht: 130 kDa

Gen-ID: 4162, 599039

UniProt: P43121