CDT2/RAMP Antikörper (AA 541-730)

Produktnummer ABIN6139847

Der Kaninchen Polyklonal Anti-CDT2/RAMP-Antikörper wurde für WB validiert. Er ist geeignet, CDT2/RAMP in Proben von Human zu detektieren.

Kurzübersicht für CDT2/RAMP Antikörper (AA 541-730) (ABIN6139847)

Target: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDT2/RAMP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-CDT2/RAMP Antikörper

Bindungsspezifität: AA 541-730

Sequenz: AEACSESRNR VKRRLDSSCL ESVKQKCVKS CNCVTELDGQ VENLHLDLCC LAGNQEDLSK DSLGPTKSSK IEGAGTSISE PPSPISPYAS ESCGTLPLPL RPCGEGSEMV GKENSSPENK NWLLAMAAKR KAENPSPRSP SSQTPNSRRQ SGKTLPSPVT ITPSSMRKIC TYFHRKSQED FCGPEHSTEL

Kreuzreaktivität: Maus, Ratte

Produktmerkmale: Polyclonal Antibodies

Aufreinigung: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 541-730 of human DTL (NP_057532.3).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB,1:500 - 1:2000

Kommentare:

HIGH QUALITY

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C. Avoid freeze / thaw cycles.

Details zu CDT2/RAMP

Target: CDT2/RAMP (DTL) (Denticleless E3 Ubiquitin Protein Ligase Homolog (DTL))

Andere Bezeichnung: DTL

Hintergrund: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL complex, also named CRL4(CDT2 complex, mediates the polyubiquitination and subsequent degradation of CDT1, CDKN1A/p21(CIP1, FBH1, KMT5A and SDE2. CDT1 degradation in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDKN1A/p21(CIP1 degradation during S phase or following UV irradiation is essential to control replication licensing. KMT5A degradation is also important for a proper regulation of mechanisms such as TGF-beta signaling, cell cycle progression, DNA repair and cell migration. Most substrates require their interaction with PCNA for their polyubiquitination: substrates interact with PCNA via their PIP-box, and those containing the 'K+4' motif in the PIP box, recruit the DCX(DTL complex, leading to their degradation. In undamaged proliferating cells, the DCX(DTL complex also promotes the 'Lys-164' monoubiquitination of PCNA, thereby being involved in PCNA-dependent translesion DNA synthesis. The DDB1-CUL4A-DTL E3 ligase complex regulates the circadian clock function by mediating the ubiquitination and degradation of CRY1.,DTL,CDT2,DCAF2,L2DTL,RAMP,Cell Biology & Developmental Biology,Cell Cycle,Ubiquitin,DTL

Molekulargewicht: 23 kDa/79 kDa

Gen-ID: 51514

UniProt: Q9NZJ0