IDUA Antikörper (AA 26-653)

Produktnummer ABIN603316

Dieses Maus Monoklonal-Antikörper erkennt spezifisch IDUA in ELISA und WB. Er zeigt eine Reaktivität gegenüber Human und wurde in 2+ Publikationen erwähnt.

Kurzübersicht für IDUA Antikörper (AA 26-653) (ABIN603316)

Target: IDUA (Iduronidase, alpha-L- (IDUA))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser IDUA Antikörper ist unkonjugiert

Applikation: ELISA, Western Blotting (WB)

Klon: 452619

Produktdetails anti-IDUA Antikörper

Bindungsspezifität: AA 26-653

Spezifität: Recognizes human Alpha-L-Iduronidase. Species sequence Homology: Mouse (79 % ), chicken (61 %).

Aufreinigung: Protein G purified

Immunogen: NS0-derived, recombinant human IDUA aa26-653.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), WB (1 - 2 μg/mL)

Usage: Suitable for use in ELISA and Western Blot. Direct ELISA: 0.5-1 μg/mL. The detection limit is ~1 ng/well. Antibody was tested using full-length, recombinant protein. Western Blot: 1-2 μg/mL. Using a colorimetric detection system, the detection limit is ~5 ng/lane under non-reducing and reducing conditions. Antibody tested in WB using the full-length recombinant protein. Chemiluminescent detection will increase sensitivity by 5-50 fold.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.4, 5 % trehalose

Handhabung: Aliquot to Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Lyophilized powder may be stored at -20°C. Stable for 1 year at -20°C. Aliquot to avoid freeze-thaw cycles. Store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Referenzen für anti-IDUA Antikörper (ABIN603316)

Gutierrez-Valdes, Häkkinen, Lemasson, de Groot, Ele-Ekouna, Guillet, Cardon, Ritala: "Improving yield of a recombinant biologic in a Brassica hairy root manufacturing process." in: Biotechnology and bioengineering, (2022) (PubMed).

Cardon, Pallisse, Bardor, Caron, Vanier, Ele Ekouna, Lerouge, Boitel-Conti, Guillet: "Brassica rapa hairy root based expression system leads to the production of highly homogenous and reproducible profiles of recombinant human alpha-L-iduronidase." in: Plant biotechnology journal, Vol. 17, Issue 2, pp. 505-516, (2019) (PubMed).

Details zu IDUA

Target: IDUA (Iduronidase, alpha-L- (IDUA))

Andere Bezeichnung: IDUA / MPS1

Hintergrund: Name/Gene ID: IDUA

Synonyms: IDUA, Alpha-L-iduronidase, MPS1, Iduronidase, alpha-L-, IDA

Gen-ID: 3425

Pathways: Glycosaminoglycan Metabolic Process