ADAM10 Antikörper (APC)

Produktnummer ABIN602624

Der Maus Monoklonal Anti-ADAM10-Antikörper wurde für FACS validiert. Er ist geeignet, ADAM10 in Proben von Human zu detektieren.

Kurzübersicht für ADAM10 Antikörper (APC) (ABIN602624)

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ADAM10 Antikörper ist konjugiert mit APC

Applikation: Flow Cytometry (FACS)

Klon: 163003

Produktdetails anti-ADAM10 Antikörper (APC)

Spezifität: Recognizes human ADAM10 (APC).

Aufreinigung: Purified

Immunogen: Mouse myeloma cell line NS0-derived recombinant human ADAM10 (aa Thr214 - Glu672, UniProt Accession #O14672).

Isotyp: IgG2b

Anwendungsinformationen

Applikationshinweise: Approved: Flo

Usage: Suitable for use in Flow Cytometry Flow Cytometry: Intracellular staining antibodies are designed for multiparameter flow cytometric analysis of cells. To stain for surface proteins (e. g. CD3, CD4, CD8) in addition to the intracellular protein, we recommend that the investigator determine whether the fixation and permeabilization steps adversely affect the surface protein. If so, surface staining of cells prior to fixation and permeabilization is recommended. For intracellular staining, cells must first be fixed and permeabilized. Use of 4 % paraformaldehyde in PBS as a fixative is recommended. Other formulations or tissue fixatives may affect the staining properties of the monoclonal antibody. For permeabilization, 0.1 % saponin in a balanced salt solution is effective in facilitating antibody entry into cells. Due to the reversible nature of cell membrane permeabilization, saponin must be included in all buffers used (i. e. both the staining and washing steps). 10 μL of undiluted product can be used to stain 1 x 10e6 cells. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 40-50 % glycerol, PBS.

Konzentration: Lot specific

Buffer: Lyophilized from 25 μg antibody, 1 mL PBS, 0.1 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C. Aliquots are stable for at least 3 months at -20°C.

Details zu ADAM10

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Andere Bezeichnung: ADAM10

Hintergrund: Name/Gene ID: ADAM10
Subfamily: Metallopeptidase M12B
Family: Protease

Synonyms: ADAM10, AD10, ADAM 10, Kuzbanian, HsT18717, CD156c, CD156c antigen, CDw156, Kuz, Kuzbanian protein homolog, MADM

Gen-ID: 102

UniProt: O14672

Pathways: Notch Signalweg, EGFR Signaling Pathway