CSPG5 Antikörper (AA 31-420)

Produktnummer ABIN601028

Der Ziege Polyklonal Anti-CSPG5-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, CSPG5 in Proben von Ratte und Maus zu detektieren.

Kurzübersicht für CSPG5 Antikörper (AA 31-420) (ABIN601028)

Target: CSPG5 (Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5))

Reaktivität: Ratte, Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser CSPG5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-CSPG5 Antikörper

Bindungsspezifität: AA 31-420

Spezifität: Recognizes Neuroglycan C, extracellular domain. In direct ELISA, this antibody shows 25 % cross-reactivity with rhNeuroglycan C. Species cross-reactivity: Mouse, rat.

Aufreinigung: Affinity purified

Sterilität: Sterile filtered

Immunogen: Purified, CHO cell-derived, recombinant mouse Neuroglycan C (rmNeuroglycan C, aa 31-420, Accession# AAH55736).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), IHC (10 μg/mL), WB (1 μg/mL)

Usage: Suitable for Immunohistochemistry ELISA and Western Blot. ELISA: 0.5-1 μg/mL. Western Blot: 1 μg/mL. Immunohistochemistry: 10 μg/mL detects Neuroglycan C in sections of adult mouse cerebellum. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at RT and blocked. with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at RT After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-goat lgG at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1 % BSA.

Kommentare:

Target Species of Antibody: Mouse

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS

Konzentration: Lot specific

Buffer: Lyophilized from a 0.2 um sterile-filtered solution in PBS, 5 % trehalose

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.

Details zu CSPG5

Target: CSPG5 (Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5))

Andere Bezeichnung: CSPG5 / Neuroglycan C

Hintergrund: Name/Gene ID: CSPG5

Synonyms: CSPG5, CALEB, Neuroglycan C, NGC

Gen-ID: 10675

UniProt: O95196

Pathways: Glycosaminoglycan Metabolic Process