CSPG5 Antikörper (Extracellular Domain)

Produktnummer ABIN601027

Dieser Schaf Polyklonal Antikörper detektiert spezifisch CSPG5 in WB, ELISA und ICC. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CSPG5 Antikörper (Extracellular Domain) (ABIN601027)

Target: CSPG5 (Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5))

Reaktivität: Human

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser CSPG5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-CSPG5 Antikörper

Bindungsspezifität: Extracellular Domain

Spezifität: Recognizes human Neuroglycan C. Shows approximately 50 % cross-reactivity with recombinant mouse Neuroglycan C in direct ELISA.

Aufreinigung: Immunoaffinity purified

Sterilität: Sterile filtered

Immunogen: CHO cell-derived rhNeuroglycan C extracellular domain.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (1 μg/mL)

Usage: Applications:Suitable for use in ELISA, Immunocytochemistry and WesternBlot. Other Applications not tested.Recommended Dilution:Direct ELISA: 0.5-1 μg/mL. The detection limit for recombinanthuman Neuroglycan C is approximately 0.3 ng/well.Western Blot: 1 μg/mLImmunocytochemistry: 10 μg/mL was used to detect NeuroglycanC in sections of adult mouse cerebellum. Cells were fixed withPBS containing 4 % paraformaldehyde for 20 minutes at roomtemperature and blocked with PBS containing 10 % normaldonkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutesat RT, After blocking, cells were incubated with dilutedLS-C123249 for 3 hours at RT followed by NorthernLights 557donkey anti-sheep lgG at RT for 1 hour. Between each step, cellswere washed with PBS containing 0.1 % BSA.Optimal dilutions to be determined by the researcher

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile 40-50 % glycerol.

Konzentration: Lot specific

Buffer: Lyophilized from a 0.2 um sterile-filtered solution in PBS, 5 % trehalose

Handhabung: Avoid repeated freezing and thawing

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.

Details zu CSPG5

Target: CSPG5 (Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5))

Andere Bezeichnung: CSPG5 / Neuroglycan C

Hintergrund: Name/Gene ID: CSPG5

Synonyms: CSPG5, CALEB, Neuroglycan C, NGC

Gen-ID: 10675

UniProt: O95196

Pathways: Glycosaminoglycan Metabolic Process