CSPG5 Antikörper (Extracellular Domain)

Produktnummer ABIN601027

Dieses Schaf Polyklonal-Antikörper erkennt spezifisch CSPG5 in WB, ELISA und ICC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CSPG5 Antikörper (Extracellular Domain) (ABIN601027)

Target: CSPG5 (Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5))

Reaktivität: Human

Wirt: Schaf

Klonalität: Polyklonal

Konjugat: Dieser CSPG5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunocytochemistry (ICC)

Produktdetails anti-CSPG5 Antikörper

Bindungsspezifität: Extracellular Domain

Spezifität: Recognizes human Neuroglycan C. Shows approximately 50 % cross-reactivity with recombinant mouse Neuroglycan C in direct ELISA.

Aufreinigung: Immunoaffinity purified

Sterilität: Sterile filtered

Immunogen: CHO cell-derived rhNeuroglycan C extracellular domain.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (0.5 - 1 μg/mL), ICC (10 μg/mL), WB (1 μg/mL)

Usage: Applications:Suitable for use in ELISA, Immunocytochemistry and WesternBlot. Other Applications not tested.Recommended Dilution:Direct ELISA: 0.5-1 μg/mL. The detection limit for recombinanthuman Neuroglycan C is approximately 0.3 ng/well.Western Blot: 1 μg/mLImmunocytochemistry: 10 μg/mL was used to detect NeuroglycanC in sections of adult mouse cerebellum. Cells were fixed withPBS containing 4 % paraformaldehyde for 20 minutes at roomtemperature and blocked with PBS containing 10 % normaldonkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutesat RT, After blocking, cells were incubated with dilutedLS-C123249 for 3 hours at RT followed by NorthernLights 557donkey anti-sheep lgG at RT for 1 hour. Between each step, cellswere washed with PBS containing 0.1 % BSA.Optimal dilutions to be determined by the researcher

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile 40-50 % glycerol.

Konzentration: Lot specific

Buffer: Lyophilized from a 0.2 um sterile-filtered solution in PBS, 5 % trehalose

Handhabung: Avoid repeated freezing and thawing

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: May be stored at 4°C for short-term only. For long-term storage and to avoid freeze-thaw cycles, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 1 year at -20°C.

Details zu CSPG5

Target: CSPG5 (Chondroitin Sulfate Proteoglycan 5 (Neuroglycan C) (CSPG5))

Andere Bezeichnung: CSPG5 / Neuroglycan C

Hintergrund: Name/Gene ID: CSPG5

Synonyms: CSPG5, CALEB, Neuroglycan C, NGC

Gen-ID: 10675

UniProt: O95196

Pathways: Glycosaminoglycan Metabolic Process