PRSS3 Antikörper

Produktnummer ABIN598236

Dieser Anti-PRSS3 Antikörper ist ein Ratte Monoklonal Antikörper zur Detektion von PRSS3 in WB und Neut. Geeignet für Human.

Kurzübersicht für PRSS3 Antikörper (ABIN598236)

Target: PRSS3 (Protease, serine, 3 (PRSS3))

Reaktivität: Human

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser PRSS3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Neutralization (Neut)

Produktdetails anti-PRSS3 Antikörper

Spezifität: Recognizes both pro and mature forms of human trypsin 3. Species sequence homology: chimpanzee: 96 % , rat: 76 %, mouse: 74 %, dog: 72 %. and chicken: 67 %. Does not cross-react with rhTrypsin-1 or rhTrypsin-2.

Aufreinigung: Protein G purified

Immunogen: NS0-derived recombinant human rhTrypsin-3.

Type of Immunogen: Recombinant protein

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Approved: Neut, WB (1 - 2 μg/mL)

Usage: Suitable for use in Neutralization and Western Blot. Neutralization: The antibody was preincubated with the enzyme at different molar ratios at RT for 30 minutes. The enzyme was then assayed with a fluorogenic peptide substrate. 50 % of the proteolytic activity was inhibited by the antibody at approximately 0.15 μg/mL (IC 50) under conditions in which the enzyme was present at 0.03 μg/mL and the substrate concentration was 10uM. Considering the molecular masses of the enzyme (26kD) and the antibody (150kD), IC 50 was achieved at approximately 1:1 Molar ratio of the antibody to the enzyme. Western blot:1-2 μg/mL. Using a colorimetric detection system, the detection limit for rhTrypsin-3 is ~25 ng/lane under non-reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Reducing conditions are not recommended.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile buffer.

Konzentration: Lot specific

Buffer: Lyophilized from PBS, 5 % trehalose

Lagerung: -20 °C

Informationen zur Lagerung: Lyophilized powder may be stored at -20°C. Aliquot and store at -20°C. Reconstituted product is stable for 1 year at -20°C.

Details zu PRSS3

Target: PRSS3 (Protease, serine, 3 (PRSS3))

Andere Bezeichnung: PRSS3 / Trypsin 3

Hintergrund: Name/Gene ID: PRSS3
Subfamily: Serine S1
Family: Protease

Synonyms: PRSS3, Brain trypsinogen, Mesotrypsin, PRSS4, Serine protease 4, Serine protease 3, TRY4, Trypsin III, Trypsin IV, Trypsinogen 4, MTG, Trypsinogen 5, Pancreatic trypsinogen III, Protease, serine, 3, Trypsin-3, Mesotrypsinogen, T9, TRY3, Trypsinogen IV

Gen-ID: 5646

UniProt: P35030